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| Registros recuperados: 29 | |
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| Ferreira,Kátia; Torres,Giovana Augusta; Carvalho,Isabelle Vilela de; Davide,Lisete Chamma. |
| The objective of this work was to compare the meiotic behavior and pollen grain viability of three species of Crotalaria. Slides for meiotic analysis were prepared by the air-drying technique. Pollen grain viability was measured by three staining procedures (Alexander's solution, tetrazolium chloride and fluorescein diacetate) and in vitro germination in a sucrose solution. Eight bivalents were observed, confirming previous reports on populations from other regions of Brazil, as well as from other countries. All species showed abnormal meiotic behavior as follows: in Crotalaria micans, cytomixis and abnormal chromosome pairing in diakinesis; in C. spectabilis, abnormal chromosome pairing in diplotene; in C. zanzibarica, shrunk nuclei in leptotene and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crotalaria; Cytogenetics; Cytomixis; Meiosis; Pollen grain viability. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009001200012 |
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| Pereira,Luciana Lopes Silva; Marcussi,Silvana; Sátiro,Lívia Cabral; Pereira,Chrystian Araujo; Andrade,Larissa Fonseca; Davide,Lisete Chamma; Santos,Custódio Donizete dos. |
| This study was conducted to evaluate the potential induction of genotoxic effects of white bean flour using the Comet assay. The test was conducted with human lymphocytes present in whole blood immediately after collection, by incubation with white bean flour in three concentrations (3.92, 9.52 and 18.18 mg/mL) at 37 ºC for 4 h followed by preparation of slides. Samples were considered positive (above 20% damage) when the damage observed to cellular DNA was higher than the negative control. No genotoxic potential was found at the doses tested. However, it would be premature to suggest absence of risk to human health of DNA damage since the exposure of cells to the extract was restricted to four hours rather than a whole cell cycle. Additionally, further... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genotoxicity; White bean flour/genotoxic effects; Comet assay/toxicological analysis of food; Food/toxicological analysis. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100012 |
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| Fajardo,Cristiane Gouvêa; Davide,Lisete Chamma; Pereira,Antônio Vander. |
| This study evaluated the structure and the volume of interphase nuclei from root meristems of the genotypes of napier grass (Pennisetum purpureum), pearl millet (Pennisetum glaucum) and hybrids resultant of such breeding. In napier grass, nuclei were areticulate. Both pearl millet and the triploid hybrid had semi-reticulate nuclei; also, the hybrid presented a small proportion (6%) of areticulate nuclei. Pearl millet had the highest averages of nuclear dimensions, such as volume, diameter and radius, followed by the interspecific hybrid and napier grass. There was no intraspecific variation for the type of nuclear structure, which indicates this feature is important for cytotaxonomic studies involving the genus Pennisetum. Results demonstrated that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Napier grass; Pearl millet; Interphase chromatin; Nuclear volume. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000500007 |
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| Tomé,Lívia Gracielle Oliveira; Silva,Adriano Bortolotti da; Pinto,Cesar Augusto Brasil Pereira; Davide,Lisete Chamma; Pereira,Dalvana Sousa; Carvalho,Carlos Roberto de. |
| ABSTRACT: The aim of the present study was to evaluate the effect of the colchicine and oryzalin anti-mitotic substances on the induction of tetraploid plants and foliar anatomy of two diploids clones of Solanum commersonii ssp. Nodal segments of Solanum commersonii subsp. commersonii Dun. and Solanum commersonii subsp. malmeanum Bitt. were treated with colchicine (3.5, 5.0, and 6.5mM; 72h) or oryzalin (10, 30, and 50µM; 24h). After the treatment with anti-mitotic substances, nodal segments were inoculated in the MS culture medium and cultivated in vitro (60 days). After in vitro cultivation, the plants were transferred to vases with the substrate Plantmax(r) and kept in the greenhouse (45 days). Plant ploidy level was assessed by flow cytometry and leaf... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Potato; Crop breeding; Flow citometry; Foliar anatomy. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001101973 |
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| Labory,Cláudia Regina Gontijo; Dias,Eustáquio Souza; Davide,Lisete Chamma; Schawan,Rosane Freitas; Wenzel,Inajá Marchizeli. |
| Com o objetivo de se estudar o comportamento nuclear durante o ciclo sexual do cogumelo Agaricus blazei , técnicas de coloração e a utilização de fluorocromos foram testadas, visando a uma padronização metodológica para estudos citogenéticos futuros. Das técnicas avaliadas neste trabalho (método de Feulgen, coloração com Giemsa e fluorescência-DAPI), a coloração com Giemsa e o fluorocromo DAPI apresentaram os melhores resultados para evidenciar núcleos. O corante Giemsa permitiu a visualização de compartimentos multinucleados. Com o DAPI, não foi possível a visualização dos septos, sendo necessária a utilização adicional de calcofluor, que tem a propriedade de corar a quitina que delimita os compartimentos das hifas. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agaricus blazei; Giemsa; DAPI; Calcofluor; Citogenética. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542003000200030 |
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| Barbosa,Sandro; Davide,Lisete Chamma; Pereira,Antônio Vander. |
| Cytogenetic studies were carried out on five accesses of interspecific hybrids of Pennisetum purpureum and Pennisetum glaucum and their parentals, which are part of the Forage Germplasm Bank of the "Centro Nacional de Pesquisa de Gado de Leite" of EMBRAPA, Brazil. The mitotic cytogenetic analysis, performed using stain by Schiff reactive, confirmed the chromosome number of 2n = 14, 28 and 21 for the accesses of P. glaucum, P. purpureum and their hybrids, respectively. For each access, descriptions of the chromosome complement and idiograms are presented which allowed to establish the karyotypic relationships between the hybrids and their parentals. Based on the total chromosomal length data it was possible to establish the karyotype classification of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Elephant grass; Pearl millet; Interspecific hybrids; Cytogenetic; Ideograms; Chromosome. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542003000100003 |
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| Techio,Vânia Helena; Davide,Lisete Chamma; Pereira,Antônio Vander; Bearzoti,Eduardo. |
| Cytotaxonomic studies were carried out on 26 accessions of Pennisetum spp. originating from the Active Germplasm Bank of Embrapa Gado de Leite-Juiz de Fora, Minas Gerais, Brazil.This study presents cytogenetics and reproductive morphological data for each one of these accessions, which allowed groupings and suggest botanical identifications to be established. The metaphases of the accessions characterized as P. purpureum confirmed the presence of 2n = 28 chromosomes that have already been described for this species. For the hybrids between P. purpureum and P. glaucum and for the hexaploids, resulting from the chromosome duplication of these hybrids, 2n = 21 and 2n = 42 chromosomes were confirmed, respectively. The wild accession identified as P. setosum... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pennisetum; Cytogenetic; Taxonomy; Germplasm; Genetic divergence. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200014 |
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| Pereira,Roselaine Cristina; Davide,Lisete Chamma; Techio,Vânia Helena; Timbó,Ana Luiza Oliveira. |
| A obtenção artificial de genótipos com duplicação cromossômica em forrageiras busca maximizar características de interesse agronômico, como valor nutricional e produção de forragem, resistência a pragas e doenças, tolerância a estresses abióticos e restauração da fertilidade de híbridos estéreis. Outro objetivo da duplicação cromossômica é a geração de variabilidade genética em espécies apomíticas, por meio da duplicação cromossômica de acessos sexuais igualando a ploidia de modo a permitir a realização de cruzamentos e a obtenção de descendentes férteis. A indução de poliploidia é feita utilizando substâncias antimitóticas, sendo a colchicina a mais amplamente usada em plantas forrageiras. Entretanto, devido a sua toxicidade, outras substâncias como... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Poliploidização; Substâncias antimitóticas; Colchicina; Herbicidas; Cafeína; Explantes. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000700023 |
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| Hallak,Angela Maria Gattás; Davide,Lisete Chamma; Souza,Itamar Ferreira. |
| Two experiments were conducted to test the allelopathic effect of sorghum (Sorghum bicolor L.) root exudates on bean (Phaseolus vulgaris L.) cell division. Research was conducted in the greenhouse of the Wistock Agricultural Research Institute of Minas Gerais State (EPAMIG) and in a laboratory of the Federal University of Lavras (UFLA). Sorghum variety BR-601 and bean variety Carioca MG were used. The exudate, called sorgoleone (SGL), was obtained by methylene chloride and acetic acid extraction from sorghum roots seven days after sowing on Petri dishes, and refrigerated until use. Solutions of 0.01, 0.05, 0.10, and 0.15 mM were prepared using Johanson solution as the SGL solvent. Seven-day-old bean seedlings grown in vermiculite in a greenhouse were... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100018 |
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| Campos,José Marcello Salabert de; Calderano,Clara de Azevedo; Pereira,Antônio Vander; Davide,Lisete Chamma; Viccini,Lyderson Facio; Santos,Marcelo de Oliveira. |
| Os objetivos deste trabalho foram estabelecer um protocolo eficiente de embriogênese somática, em híbridos triploides entre capim elefante (Pennisetum purpureum Schumach.) e milheto (P. glaucum (L.) R. Br.), e avaliar por citometria de fluxo a estabilidade genômica das plantas obtidas in vitro. A embriogênese somática e a regeneração das plantas foram estabelecidas a partir de embriões zigóticos maduros de híbridos entre capim elefante e milheto. Foram testados quatro tratamentos com 2,4 ácido diclorofenoxiacético (2,4 D), nas concentrações 0, 1, 2 e 3 mg L-1, para indução de calos embriogênicos, e dois tratamentos com inositol a 1 e 2 g L-1, para regeneração das plantas. Os tratamentos foram dispostos em delineamento inteiramente ao acaso. A combinação... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pennisetum glaucum; Pennisetum purpureum; Cultura de tecidos. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000100006 |
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| Mauri,Janaína; Pereira,Daniele Laís; Silva,Gabrielle Avelar; Davide,Lisete Chamma; Techio,Vânia Helena; Souza Sobrinho,Fausto; Pereira,Fabricio José. |
| ABSTRACT: The digestibility potential of leaves from forages depends on the amount of nutrition in their tissues, with low lignin deposition in the cell wall, mainly parenchyma and phloem. This research evaluated the leaf structure of different Urochloa genotypes and discussed its potential for evaluating digestibility. The cultivars U. brizantha, U. decumbens, U. ruziziensis and three clones of U. ruziziensis (1, 95 and 97), which are under development in breeding programs, were evaluated. Plants were grown under the recommended culture conditions for the Urochloa species. Plants were cut 60 days after sowing, and leaves were collected at 15 days of regrowth. Leaves were fixed in FAA 70 and further stored in 70% ethanol until being submitted to the usual... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Digestibility; Forage production; Leaf anatomy; Sclerenchyma; Lignin. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000400401 |
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| Timbó,Ana Luiza de Oliveira; Pereira,Roselaine Cristina; Santos,Vanderley Borges dos; Souza Sobrinho,Fausto; Davide,Lisete Chamma. |
| Flow cytometry allows to estimate the DNA content of a large number of plants quickly. However, inadequate protocols can compromise the reliability of these estimates leading to variations in the values of DNA content the same species. The objective of this study was to propose an efficient protocol to estimate the DNA content of Brachiaria spp. genotypes with different ploidy levels using flow cytometry. We evaluated four genotypes (B. ruziziensis diploid and artificially tetraploidized; a tetraploid B. brizantha and a natural triploid hybrid), three buffer solutions (MgSO4, Galbraith and Tris-HCl) and three species as internal reference standards (Raphanus sativus, Solanum lycopersicum e Pisum sativum). The variables measured were: histogram score (1-5),... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brachiaria ruziziensis; Brachiaria brizantha; Colchicine; Nuclear DNA content. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000600001 |
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| Santos,Fernanda Motta da Costa; Torres,Giovana Augusta; Techio,Vânia Helena; Pereira,Antônio Vander; Davide,Lisete Chamma. |
| Abstract: The objective of this work was to evaluate, by genomic in situ hybridization (GISH), pairing configurations as potential indicators of recombination between chromosomes of different parental genomes, in two interspecific hybrids (elephant grass x pearl millet) artificially polyploidized. Anthers from young flower buds were used in the chromosomal preparations. The genomic probe was prepared with pearl millet DNA and labeled with biotin-16-dUTP by the nick translation reaction. Blocking DNA was prepared with genomic elephant grass DNA. The homoeologous intergenomic pairing, observed in the two hybrids, indicates the possibility of recombination between chromosomes of the parental genomes. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pennisetum glaucum; Pennisetum purpureum; In situ hybridization; Intergenomic pairing; Interspecific hybridization. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000900814 |
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| Registros recuperados: 29 | |
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